Turkish Neurosurgery 2017 , Vol 27 , Num 1
The Axon Protective Effects of Syringic Acid on Ischemia/Reperfusion Injury in a Rat Sciatic Nerve Model
Mehmet TOKMAK1,Muserref Hilal SEHITOGLU2, Yasemin YUKSEL3,Mustafa GUVEN4,Tarik AKMAN4,Adem Bozkurt ARAS4,Umut YAKA1,Cengiz GOMLEKSIZ1,Serdar Baki ALBAYRAK1,Murat COSAR4
1Medipol University, Faculty of Medicine, Department of Neurosurgery, Istanbul, Turke
2Canakkale Onsekiz Mart University, Faculty of Medicine, Department of Medical Biochemistry, Canakkale, Turkey
3Afyon Kocatepe University, Faculty ofMedicine, Department of Histology and Embryology, Afyon, Turkey
4Canakkale Onsekiz Mart University, Faculty of Medicine, Department of Neurosurgery, Canakkale, Turkey
DOI : 10.5137/1019-5149.JTN.14656-15.5 AIM: In the relevant literature, there is no experimental study that investigated the axon protective effects of syringic acid- a polyphenol compound- with an anti-oxidant capacity on ischemia/reperfusion injury.

MATERIAL and METHODS: The rats were randomly divided into four groups: Control group (no medication or surgical procedure), Sham group, Syringic acid group, and Methyprednisolone (MP) Group. Ischemia was achieved by abdominal aorta clamping and all animals were sacrificed 24 hours after ischemia. Harvested sciatic nerve segments were investigated histopathologically and for tissue biochemistry.

RESULTS: Ischemic fiber degeneration scores were found significantly lower in syringic acid and MP groups than sham group. Additionally, apoptosis-related cysteine peptidase caspase-3 immunostaining scores were lower in syringic acid and MP groups. Biochemically, superoxide dismutase and nuclear respiratory factor 1 values were significantly higher in syringic acid group compared to those of control and sham groups while malondialdehyde levels were significantly lower in the syringic acid group.

CONCLUSION: Syringic acid reduces oxidative stress and axonal degeneration in rat sciatic nerve after ischemia/reperfusion injury. Therefore, syringic acid may play a role in the treatment of peripheral nerve injuries due to ischemia/reperfusion. Keywords : Axon, Sciatic nerve, Syringic acid, Methylprednisolone, Reperfusion, Malondialdehyde, Nuclear respiratory factor 1, Superoxide dismutase