Turkish Neurosurgery 2021 , Vol 31 , Num 3
Analysis of Mitochondrial DNA Control Region D-Loop in Gliomas: Result of 52 Patients
Sirin K. YUKSEL1,Koray OZDUMAN2,Engin YILMAZ3,M. Necmettin PAMIR2, Cemaliye B. AKYERLI3
1Acibadem Mehmet Ali Aydinlar University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkey
2Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Neurosurgery, Istanbul, Turkey
3Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Medical Biology, Istanbul, Turkey
DOI : 10.5137/1019-5149.JTN.29805-20.2 AIM: To investigate the effect of mitochondrial DNA (mtDNA) variants mainly in D-loop on glioma biology.

MATERIAL and METHODS: Sanger sequencing of D-loop (15971?16451 bp) for 52 glioma patients was performed and the variations were statistically analyzed for gender, WHO classification, morphological grade, IDH/TERT status.

RESULTS: Total of 122 variations (51 unique) were identified in 52 patients. C16223T, T16189C, T16311C and T16126C variants were frequently detected. The total variation number was statistically non-significant among the analyzed categories. When individual variants were considered, T16311C and T16224C were statistically significant for WHO classification (p=0.033), morphological grade (p=0.036) and gender (p=0.039), respectively.

CONCLUSION: Total variation number in D-loop was not found to be related with clinical variables. Our data suggests that individual variants may play a critical role in glioma biology. Keywords : D-loop, Glioma, Mitochondrial DNA, mtDNA variations, Mutations

Corresponding author : Cemaliye BOYLU AKYERLI, cemaliye.boylu@acibadem.edu.tr