Turkish Neurosurgery
Postlaminectomy bone and scar formations in presence of Ankaferd Blood Stopper and bitter melon (Momordica charantia); an experimental study
Enis Kuruoglu1, Mehmet Emin Onger2, Abdullah Hilmi Marangoz1, Suleyman Emre Kocacan3, Cengiz Cokluk1, Suleyman Kaplan2
1Ondokuz Mayıs University, Neurosurgery, Samsun,
2Ondokuz Mayıs University, Histology and Embryology, Samsun,
3Ondokuz Mayıs University, Physiology, Samsun,
DOI: 10.5137/1019-5149.JTN.16263-15.1
Aim:In the present study, a quantitative model of post-laminectomy in rats was designed. Concurrently, the effects of Momordica charantia (MC) and Ankaferd blood stopper (ABS) were evaluated.Material and Methods:Eighteen adult Wistar albino rats underwent lumbar laminectomy at L2-L3 vertebral levels, and were randomly assigned to one of three groups of six rats each. The treatment group received MC and ABS treatment and the Control group was untreated. Rats were sacrificed 4 weeks after treatment. Then; the lumbar spine excised as a block, fixed and decalcified. Sections were stained with hematoxylin and eosin (H&E) and Masson’s trichrome were used to evaluate peridural fibrosis (PF), new bone, and vascular proliferation.Results:According to the findings; the total volume of new bone in the MC group was significantly increased in comparison to the Control group (P<0.05). Also; there was highly significant increase in terms of the total volume of fibrous tissue in the MC and ABS groups when compared with the Control group (P<0.01). Besides; there was highly significant difference between the MC and the Control groups (P<0.01) in point of total volume of vessel.Conclusion:This experimental study shows that both MC and ABS are not convenient to block PF formation and also MC may promote new bone formation and angiogenesis after lumbar laminectomy in rats.
Corresponding author : Mehmet Emin Onger