Turkish Neurosurgery
The Effects of Stereotactic Cerebroventricular Administration of Albumin, Mannitol, Hypertonic Sodium Chloride, Glycerin and Dextran in Rats with Experimental Brain Edema
Tuncay Ateş1, Yurdal Gezercan2, Güner Menekşe3, Yusuf Türköz4, Hakan Parlakpınar5, Ali İhsan Ökten2, Yener Akyuva6, Selami Çağatay Önal6
1Cukurova Dr. Askim Tufekci State Hospital, Department of Neurosurgery, Adana,
2Adana Numune Training and Research Hospital, Department of Neurosurgery, Adana,
3Ankara Training and Research Hospital, Department of Neurosurgery, Ankara,
4Inonu University Faculty of Medicine, Department of Biochemistry, Malatya,
5Inonu University Faculty of Medicine, Department of Pharmacology, Malatya,
6Inonu University Faculty of Medicine, Department of Neurosurgery, Malatya,
DOI: 10.5137/1019-5149.JTN.17231-16.1
Aim:We aimed to evaluate the effects of cerebroventricular administration of hyperoncotic/hyperosmotic agents on edematous tissue in rats with experimental head trauma. Material and Methods:The study included 54 female Sprague-Dawley rats with weights ranging between 200-250 g. Six experimental groups were examined with each group containing 9 rats. All rats were exposed to head trauma, and treatment groups were administered 2 microliters of one of the drugs (albumin, mannitol, hypertonic sodium chloride (NaCl),glycerin and dextran) 6, 12 and 24 hours after the trauma via the cerebroventricular route and using a stereotactic device. Rats were sacrificed 48 hours after the trauma, and brain tissues were extracted without damage. Biochemical analyses including reduced glutathione (GSH), nitric oxide (NO), malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), and interleukin 1 beta (IL-1-β) were performed on the injured left hemisphere. Results:Compared with the control group, the albumin, mannitol, 3% NaCl and glycerin treatment groups revealed dramatic increases in GSH levels (p<0.001). Levels of MDA, which is the end-product of brain edema and lipid peroxidation, failed to show a statistically significant decrease, but there was a decreasing trend observed in the inter-group comparisons. NO levels were also decreased in the 3% NaCl treatment group. An analysis of TNF-α and IL-1β, two proinflammatory cytokines associated with the trauma, revealed that IL-1β decreased significantly in all treatment groups (p=0.001), whereas no significant difference was detected in TNF-α levels. Conclusion:Cerebroventricular administration of hyperoncotic/hyperosmotic agents provide substantial effects on the treatment of brain edema
Corresponding author : Güner Menekşe