Turkish Neurosurgery
Induction of human glioma tumor in Sprague-Dawley rat with intact immune system
Zahra Abdi2, Hossien Eskandary3, Seyed Noureddin Nematollahi-Mahani1
1anatomy, anatomy, Kerman,
2anatomy, anatomy, Zanjan,
3Neuroscience Research Center, Kerman,
DOI: 10.5137/1019-5149.JTN.17260-16.1

Aim:Glioblastoma multiforme (GBM) is an aggressive brain tumor type in humans. The median survival of patients is a year after diagnosis. So, development of animal model is a necessary for the advances in the treatment of GBM. The aim of this study was to investigate the capability of human glioma cells in inducing glioma tumors in rats with intact immune system. Material and Methods:The U87 cells implanted in the frontal lobe of rats without suppressing of the immune system. We used the magnetic resonance imaging (MRI), Hematoxylin-Eosin (H&E) and, Immunohistochemical (IHC) staining to assess characteristic of tumor. Results:In 10 and 14 days of tumor inoculation, MRI images contained tumor areas in the brain. All tumor-bearing rats developed tumors, the rats retained important morphology and histology characteristics of human glioma. Animals mimic glioblastoma characteristic, such as mitotic activity, invasion, neovascularization, necrosis and pseudopalisading cells. IHC staining revealed tumor growth and progression in the tumor-bearing rats. Conclusion:This model is a standard test system for studying the tumor phenotype, genotype, and for evaluating the efficacy of anti-cancer agents. It is reliable, simple, inexpensive, and easily reproducible, which it may be the way to preclinical studies.

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