Turkish Neurosurgery
Is Chronic Curcumin Supplementation Neuroprotective Against Ischemia For Antioxidant Activity, Neurologic Deficit Score, Or Neuronal Apoptosis In An Experimental Stroke Model?
SERDAR ALTINAY1, MURAT CABALAR2, CIHAN ISLER3, FUNDA YILDIRIM4, DUYGU SULTAN CELIK5, OGUZHAN ZENGI6, ABDURRAHIM TAS7, AHMET GULCUBUK4
1SELCUK UNIVERSITY MEDICAL FACULTY, PATHOLOGY, KONYA,
2ISTANBUL BAKIRKOY DR. SADI KONUK TRAINING AND RESEARCH HOSPITAL, NEUROLOY, ISTANBUL,
3ISTANBUL UNIVERSITY CERRAHPASA MEDICAL FACULTY, NEUROSURGERY, ISTANBUL,
4ISTANBUL UNIVERSITY VETERINARY FACULTY, VETERINARY, ISTANBUL,
5ISTANBUL BAGCILAR TRAINING AND RESEARCH HOSPITAL , EXPERIMENTAL RESEARCH, ISTANBUL,
6ISTANBUL BAGCILAR TRAINING AND RESEARCH HOSPITAL , BIOCHEMISTRY, ISTANBUL,
7ISTANBUL BAGCILAR TRAINING AND RESEARCH HOSPITAL , NEUROSURGERY, ISTANBUL,
DOI: 10.5137/1019-5149.JTN.17405-16.0
Aim:To investigate the neuroprotective effect of chronic curcumin supplementation on the rat forebrain prior to ischemia and reperfusion Material and Methods:Forebrain ischemia was induced by bilateral common carotid artery occlusion for 1 hour, followed by reperfusion for 72 hours. Older rats were divided into five groups: I, 300 mg/kg oral curcumin for 21 days before ischemia and 300 mg/kg intraperitoneal curcumin after ischemia; II, 300 mg/kg intraperitoneal curcumin after ischemia; III, 300 mg/kg oral curcumin for 21 days before ischemia; IV, only ischemia; V:, sham-operated. The forebrain was rapidly dissected for biochemical parameter assessment and histopathology.Results:In forebrain tissue, enzyme activities of superoxide dismutase, glutathione peroxidase, and catalase were significantly higher in Group I than Groups II or III (p < 0.05 while xanthine dehydrogenase and malondialdehyde enzyme activities and concentrations of interleukin-6 and TNF-alpha were significantly lower in Group I compared to Groups II and III (p < 0.05). A significant reduction in neurological score was observed after 24 and 72 h in the curcumin-treated groups compared with the ischemic group. We also found a marked reduction in apoptotic index after 72 h in the groups receiving curcumin. Significantly more TUNEL-positive cells were observed in the ischemic group compared to those treated with curcumin.Conclusion:We demonstrated the neuroprotective effect of chronic curcumin supplement on biochemical parameters, neurologic scores and apoptosis following ischemia and reperfusion
Corresponding author : SERDAR ALTINAY