Turkish Neurosurgery
Topical Application of Cyclosporine Reduces Epineurial Fibrosis: Gross Postsurgical, Histopathological and Ultrastructural Analysis in a Rat Sciatic Nerve Model
Nuri Eralp Çetinalp1, Serdar Baki Albayrak2, Özgür İsmailoğlu3, Nail Çağlar Temiz4, İlker Solmaz4, Gamze Tanrıöver5, Necdet Demir5
1Çukurova University, Neurosurgery, Adana,
2Medipol University, Neurosurgery, İstanbul,
3Süleyman Demirerl University, Neurosurgery, Isparta,
4Gülhane Military Academy, Neurosurgery, Ankara,
5Akdeniz University, Neurosurgery, Antalya,
DOI: 10.5137/1019-5149.JTN.17871-16.1

Aim:To investigate the antiscarring potential of topical cyclosporine on rat sciatic nerves.Material and Methods:Both sciatic nerves were exposed in 24 adult male albino Wistar rats, and an abrasion injury was made on the biceps femoris close to the sciatic nerve. Cotton pads soaked with cyclosporine (5 mg/mL) and saline (0.9% NaCl) were placed around the nerves for 10 minutes in the experimental group and control group, respectively. All rats were sacrificed 8 weeks later and the sciatic nerves were examined. Epineural adhesions were assessed using light and electron microcopy. Quantitative histologic parameters, epineurial thickness, and scar density were evaluated in the histologic investigation.Results:Significantly fewer epineurial adhesions were observed in the cyclosporine group in the postsurgical assessment, and the histopathologic and ultrastructural examination of the nerve segments than in the controls. The cyclosporine-treated animanls had a statistically significant reduction in the density and quantity of epineurial scarring compared with the controls.Conclusion:Topical cyclosporine effectively reduced epineurial scar formation on rat sciatic nerves.

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