Turkish Neurosurgery
Polymorphisms in the matrix metalloproteinase-9 promoters and susceptibility to glial tumors in Turkey
Mahmut Özden1, Salim Katar2, Hakan Hanımoğlu1, Mustafa Onur Ulu3, Cihan İşler3, Oğuz Baran2, Veysel Antar2, Cumhur Gökhan Ekmekci4, Mehmet Yaşar Kaynar1
1Florence Nightingale Hospital, Neurosurgery Clinic, İstanbul,
2Istanbul Research and Training Hospital, Neurosurgery Clinic, İstanbul,
3Istanbul University, Cerrahpasa Medical Faculty, Department of Neurosurgery, İstanbul,
4Memorial Hospital, Genetics, İstanbul,
DOI: 10.5137/1019-5149.JTN.17960-16.1

Aim:Evidences suggest an association between MMP-9 functional gene polymorphisms with several tumors. The aim of this study was to investigate the possible role of single-nucleotide polymorphisms (SNP) at MMP-9 R279Q A/G, P574R G/C and R668Q G/A and R668Q (rs17577) genotypes with glial tumors in Turkey.Material and Methods:The present series consisted of tissue samples obtained from 100 cancer-free controls and 100 patients who had undergone glial tumor resection from 2007 to 2011 at the Cerrahpasa Medical Faculty of Istanbul University. Blood samples were collected to extract genomic DNA of each subject by polymerase chain reaction (PCR) and DNA sequencing. The genotypes of MMP-9 P574R, R279Q and R668Q SNPs were determined by using the PCR-RFLP assay. Genotypic distributions between patient and control groups were compared for correlations with glial tumor occurrence. Results:SNPs in MMP-9 were not found to be significantly associated with glial tumor risk among participants except R279Q (G-G) which showed high risk only in multivariate analysis (ORadjusted,3.15 95%CI, 1.10-9.01). The comparisons between the grade of tumor and the genotypic polymorphisms also showed no significant associations in case group (all p > 0.05). Conclusion:The current study has shown significant association between the R279Q G/G polymorphism association with formation of glial tumor in advanced age. Changed protein features may cause triggering some of the subcellular mechanisms that may have role activating oncogenic processes by years. These data add to the growing epidemiological and experimental evidence that MMP-9 may play a role in glial tumors.

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