Aim:Glioblastoma multiforme (GBM) is one of the malignant brain tumors, which occurs most frequently. Despite advances in therapy techniques, is the cure of GBM a major concern. Accordingly, there is a lot of interest in devising novel approaches, such as stem cell therapy, to treat patients with GBM. The aim of this study is to investigate the effects of human bone marrow stem cells (BMSCs) as well as human olfactory ensheathing cells (OECs) on outgrowth of U87 glioma in rats.Material and Methods:OECs and BMS cells were obtained from the volunteers. After verification of the stem cell type by flowcytometry and immunocytochemistry (ICC), cells were labeled and injected into human glioma-bearing rats. The magnetic resonance imaging (MRI), Hematoxylin and Eosin (H&E), and Immunohistochemistery (IHC) were utilized to assess properties of the groups.Results:We suggested the extensive migration and homing of the OECs and BMS cells towards tumor area. H&E and IHC staining indicated that the grafted OECs survived and prevented to development of glioma. Whereas, BMS cells supported of proliferation and new vessels formation, and metastasis in glioma tissue. Conclusion:Our findings provide the evidence that OECs and BMS cells can the transit of the BBB and reach to glioma mass. Therefore, this approach can be a potentially powerful method for the delivery of therapeutic agents to malignant brain tumors. In addition to these cells may be genetically modified that specifically expressed tumor-suppressive factors.