Aim:Creatine is an endogenous molecule synthesized in liver, kidney and pancreas from glycin and arginine which is important for mitochondrial metabolism. It is widely used as a supplement for improving muscle mass and function for many years. As it is expected to prevent apoptosis and diminish oxidative stress it is also studied in a number of neurodegenerative diseases for its beneficial effect in recent years. We studied the effect of creatine on the peripheral nerve injury in an experimental rat crush injury model to obtain ultrastructural evidence.Material and Methods:Animals were randomly divided in to 3 groups having 5 animals each: Group 1; the control group, Group 2; the trauma group, Group 3; the trauma + 2 g/kg creatine monohyrate administered group. The first group served as sham control. In the group 2 and group 3, sciatic nerves of the rats received crush injury using clips. After trauma, in the third group daily 2 g/kg creatine monohydrate was administered via gavage. Nerve samples were obtained at 28 day after trauma for light and electron microscopic evaluation. Results:Our comparative analysis results suggest a possible positive effect of creatine supplement on peripheral nerve regeneration as statistical analysis revealed significant differences between group 2 and group 3. Though our finding does not represent a miracle of regenerative support, beneficial usage of creatine is documented in the present study. Conclusion:Our results suggest that creatine supplement helps to diminish the harmful effects of crush injury which is also supported by electron microscopy findings.