Turkish Neurosurgery
Differential Expression of microRNAs in Medulloblastoma and the Potential Functional Consequences
Yuting Zhang1, Lusheng Li2, Ping Liang2, Xuan Zhai2, Yingliang Li2, Yudong Zhou2
1Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China, China International Science and Technology Cooperation base of Child Development and Critical Disorders, Radiology, Chongqing,
2Children’s Hospital of Chongqing Medical University,Chongqing Key Laboratory of Pediatrics., Neurosurgery, Chongqing,
DOI: 10.5137/1019-5149.JTN.19379-16.2
Aim:To identify differentially expressed microRNAs in medulloblastoma, and to investigate their biological function. medulloblastomda farklı olarak ifade microRNA tespit etmek ve bunların biyolojik fonksiyonlarını araştırmak.Material and Methods:Differentially expressed microRNAs were identified using gene chips, and significantly different microRNAs were selected for verification using real time quantitative PCR. Potential target genes and their biological pathways were predicted by bioinformatics software. Farklı eksprese mikroRNAlar gen yongaları kullanılarak belirlendi ve önemli ölçüde farklı mikroRNAlar gerçek zamanlı kantitatif PCR ile doğrulanması için seçildi. Potansiyel hedef genler ve bunların biyolojik yollar biyoinformatik yazılım tarafından tahmin edilmiştir.Results:Our analysis identified two microRNAs, hsa-miR-208a-3p and hsa-miR-1207-5p, which were significantly downregulated in medulloblastoma. Bioinformatics analysis identified potential target genes in the Wnt and MAPK signaling pathways, including NLK, RAPGEF2, CACNA2D1, DUSP3, MAPK8IP3. Analizler, iki microRNA, hsa-miR-208a-3p ve önemli ölçüde medulloblastomda olarak azaldı hsa-miR-1207-5p belirlendi. Biyoinformatik analiz NLK, RAPGEF2, CACNA2D1, DUSP3, MAPK8IP3 dahil Wnt ve MAPK sinyal yollar, potansiyel hedef genleri tespit etti.Conclusion:Downregulation of hsa-miR-208a-3p and hsa-miR-1207-5p may be involved in the occurrence of medulloblastoma, through modulations of the Wnt and MAPK signaling pathways. hsa-miR-208a-3p ve hsa-miR-1207-5p baskılanması Wnt modülasyon ve MAPK sinyal yollar aracılığıyla, medulloblastom oluşumunda rol oynayabilir.
Corresponding author : Lusheng Li