Turkish Neurosurgery
The Effect of Rosmarinus Officinalis and Chemotherapeutic Etoposide on Glioblastoma (U87 MG) Cell Culture
Meryem Damla Özdemir1, Dilek Göktürk2
1Adana Science and Technology Universitry, Graduate School of Natural and Applied Sciences, Department of Nanotechnology & Engineering Sciences, Adana,
2Adana Science and Technology University, Faculty of Engineering and Natural Sciences, Department of Bioengineering, Adana ,
DOI: 10.5137/1019-5149.JTN.20401-17.3

Aim:Glioblastoma (GBM) is the most invasive and common type of brain cancer with very poor prognosis. One of the drugs administered for GBM pharmacotherapy is etoposide (VP-16), which belongs to the topoisomerase inhibitor family. It can be used in combination with other drugs or chemicals to avoid high dose toxicities or augment its effect at lower doses. In this study we aimed to investigate whether high dose toxicities of etoposide can be overcome when used in combination with a natural compound named Rosmarinus Officinalis.Material and Methods:The impact of Rosmarinus Officinalis in combination with etoposide on GBM U87 MG cells and Mouse Embryonic Fibroblast (MEF) cells was investigated. Both neutral red and 3-(4, 5-Dimethylthiazol-2-Yl)-2, 5-Diphenyltetrazolium Bromide (MTT) assays were employed to gauge cell viability. Results:We observed that increased quantities of Rosmarinus Officinalis induced MEF cell proliferation while it inhibited the survival of GBM cells. Our results indicate that Rosmarinus Officinalis did not affect the cytotoxicity of etoposide on GBM cell cultures. In contrast, in the MEF cell cultures Rosmarinus Officinalis induced proliferation and diminished the impact of etoposide.Conclusion:Rosmarinus Officinalis offers hope for developing new cancer treatment strategies. However, further studies are needed to verift these results.

Corresponding author : Dilek Göktürk