Turkish Neurosurgery
Effects of Zinc Oxide on mRNA Expression of Genes in Brain Tissue of Rats
inan gezgin1, cem öziç2
1Dr.Ersin Aslan Eğitim ve Araştırma Hastanesi, Neurosurgery, Gaziantep,
2Kafkas University, Faculty of Medicine , Medical Biology, Kars,
DOI: 10.5137/1019-5149.JTN.21557-17.4
Aim:Zinc oxide nanoparticles are flexible platforms for biotechnology and biomedical applications. To investigate the expression level of the DR4, DR5, OPG, DcR1 and DcR2 in brain tissue.Material and Methods:The expression of DR4, DR5, OPG, DcR1, DcR2 and β-actin were measured through RT-PCR and western blotting in brain tissues of all three groups. The mRNA expression levels of DR4, DR5, OPG, DcR1 and DcR2 was investigated by using the RT-PCR and western blotting methods.Results:Thirty rats in the three groups were used in order to determine of DR4, DR5, OPG, DcR1, DcR2 and β-actin (control) genes expression. The DR4, DR5, OPG, DcR1, DcR2 genes showed a decrease in gene expression levels when they were analyzed by RT-PCR and western blotting in ZnO-NP group.Conclusion:Overexpression of DR4, DR5, OPG, DcR1 and DcR2 was observed in brain tissues. The increased expression of the DR4, DR5, OPG, DcR1 and DcR2 may play important role in ZnO-NP of brain tumors.
Corresponding author : inan gezgin