Turkish Neurosurgery
In vitro activity of linezolid, daptomycin and N-acetylcysteine agents on Staphylococcus aureus biofilms in the ventriculoperitoneal shunt model
Tuba Kuruoglu1, Mehmet Onger2, Derya Bayirli3, Enis Kuruoglu4
1Ondokuz Mayıs University, Histology and Embryology, Samsun,
2Ondokuz Mayis University, Infectious Diseases, Samsun,
3Gaziosmanpasa Hospital, Infectious Diseses, Istanbul,
4Ondokuz Mayis University, Neurosurgery, Samsun,
DOI: 10.5137/1019-5149.JTN.22470-18.1

Aim:In this study, the effect of N-acetylcysteine (NAC) alone and in combination with linezolid (LIN) and daptomycin (DAPT) was examined on methicillin-sensitive Staphylococcus aureus (MSSA) biofilm formation. Material and Methods:Twelve groups (each containing six molds) of standard ventriculoperitoneal shunts were infected with MSSA. By using microbiological and electron microscopic evaluation methods, NAC was evaluated, alone and in combination with DAPT and LIN, in terms of preventing and eliminating biofilm capacity. The effect of NAC alone and in combination with DAPT and LIN were shown by microbial counts and electron microscopic observation.Results:The results showed no significant difference in biofilm formation in shunts after different antibiotic treatments. However, the combination of NAC and DAPT had the highest bactericidal effects of all the groups.Conclusion:The resistance of bacteria and the dose-dependent effects of antibiotics can be considered.

Corresponding author : Mehmet Onger