Turkish Neurosurgery
miR-24 reduces serum lipid levels and inhibits brain tissue cells apoptosis of rats with cerebral infarction
Qiyong Jiang1, Dawei Shen2, Yihua Wang1, Dezhang Huang1, Zhigang Wang1
1Qilu Hospital of Shandong Universety, Department of Neurosurgery, Qingdao City,
2Yidu Central Hospital of Weifang, Department of Neurosurgery, Qingzhou City,
DOI: 10.5137/1019-5149.JTN.23127-18.3
Aim:To study miR-24 effects on cerebral infarction in rats.Material and Methods:A rat middle cerebral artery occlusion model (MCAO) was constructed. Intracerebroventricular stereotactic injection of miR-24 agomir/antagomir was performed in the rat MCAO model. According to different experiences, rats were divided into normal, sham, MCAO, miR-24 agomir and miR-24 antagomir groups. Serum TCH, HDL and TG levels were detected. Results:Comparing the normal and sham groups, we observed decreased relative miR-24 expression (P < 0.05) and increased cerebral infarction area percentage, apoptotic cells and relative caspase-3 protein expression (P < 0.05) in the MCAO, miR-24 agomir and miR-24 antagomir groups. TC, TG and HDL-C levels of the MCAO and miR-24 antagomir groups were higher than those of normal and sham groups (P < 0.05). Compared with the MCAO group, increased relative miR-24 expression (P < 0.05) and decreased TC, TG and HDL-C levels, cerebral infarction area percentage, number of apoptotic cells and caspase-3 expression (P < 0.05) were found in the miR-24 agomir group, contrasting with the observations from the miR-24 antagomir group. Conclusion:miR-24 reduced serum TCH, HDL and TG levels and inhibited brain tissue cell apoptosis in rats with cerebral infarction.
Corresponding author : Zhigang Wang