Aim:Deep brain stimulation of the subthalamic nucleus in patients with Parkinson’s disease (PD) can be performed with or without microelectrode recording (MER). One of the reasons not to use MER is that it increases operation time. Here, we have investigated the effect of performing of MER on operation time. Material and Methods:The time spent for MER was calculated for the first and second side in twenty-four patients with PD. The number of microelectrodes used on each trajectory for the first and second side and the permanent electrode implantation percentage on each trajectory for the first and second side were calculated as well.Results:The time spent for MER on average was 23.4 ±6.2 min., 17.4 ±6.5 min., and 41.2 ±6.3 min. for the first side, second side and total procedure, respectively. In 75% of the patients the final electrode was implanted at the planned target for the first side and in 61% on the second side.Conclusion:MER extends operation time of DBS. However, it provides real-time information during surgery on the neurophysiological location of the electrodes and facilitates the choice for an alternative target if the planned target does not produce satisfying results.