Aim:Migraine headaches are a common and significant issue experienced by women during pregnancy. However, treating migraines during pregnancy and post-pregnancy is challenging because of the risks that migraine medications pose to the fetus and infant. Few studies have investigated the effect of triptans during pregnancy, and controlled studies are not available. Our research aimed to investigate the impact of rizatriptan on neural tube development using early chick embryos as a model organism.Material and Methods:A total of 36 pathogen-free Leghorn chicken eggs were selected and categorized in three groups: sham, therapeutic, and supratherapeutic. After 24 h, the eggs were opened and injected with sterile drugs, then reclosed using plastic tape. After a period of 72 h, the eggs were opened and assessed using the Hamburger–Hamilton chick embryology classification method. TUNEL staining was used to identify apoptosis, and hematoxylin-eosin staining was used to investigate neural tube closure.Results:Treatment with rizatriptan significantly slowed down neural tube development. The supratherapeutic group showed neural tube closure defects.Conclusion:Rizatriptan had a negative effect on neural tube closure. Further research is needed to identify a safe and effective drug for treating migraines during pregnancy.