Turkish Neurosurgery
Activation of Signal transducer and activator of transcription 3 signaling attenuates neurogenesis in intracerebral hemorrhage rat model
Li Haitao3, Huajun Zhou 1
1China Three Gorges University, Institute of Neurology, Yichang,
2China Three Gorges University, Department of Neurology, The First College of Clinical Medical Sciences, Yichang,
3China Three Gorges University, Department of radiology, The First College of Clinical Medical Sciences, Yichang,
DOI: 10.5137/1019-5149.JTN.23665-18.1

Aim:Aim Neurogenesis plays an important role in promoting in neurologic function after intracerebral hemorrhage (ICH). Signal transducer and activator of transcription 3 (STAT3) activation is reported to negatively regulate neurogenesis. Previous studies have been demonstrated that activation of STAT3 signaling exerted detrimental effect on ICH. Here, the effect of STAT3 signaling on neurogenesis following ICH was investigated.Material and Methods:ICH model was induced by stereotactic injection of 100 µL autologous blood into right globus pallidus. AG490 (0.25 mg/kg) was injected into the lateral ventricle after ICH to block STAT3 signaling. Brains were perfused to identify proliferating cell nuclear antigen (PCNA)+/ doublecortin (DCX) + nuclei. The expression of p-STAT3 (Tyr 705) was evaluated by immunohistochemistry and western blot, respectively.Results:Enhanced p-STAT3 (Tyr 705) positive cells, which were expressed in neurons, were detected in the peri-hematomal region. Increases of DCX-positive cells and PCNA+/DCX+ nuclei were detected after ICH. Blocking STAT3 signaling with AG490 infusion increased the numbers of DCX-positive cells and PCNA+/DCX+ nuclei after ICH. Conclusion:These results suggest that activation of STAT 3 signaling attenuates ICH-induced neurogenesis and may delay the recovery following hemorrhagic cerebral injury.

Corresponding author : Li Haitao