Turkish Neurosurgery
The Protective Role of Hippophae rhamnoides L. on Rat Brain and Liver Tissues Exposed to Cold Plus Immobilization Stress Model
Nensi Cavak1, Mustafa Koray Gümüştaş1, Sema Demirci Çekiç2
1Istanbul University, Cerrahpasa Faculty of Medicine, Department of Medical Biochemistry, İstanbul,
2Istanbul University, Faculty of Engineering, Department of Chemistry, İstanbul,
DOI: 10.5137/1019-5149.JTN.23766-18.3

Aim:This study aimed to investigate the protective role of Hippophae rhamnoides L. (Sea buckthorn, SBT) in cold plus immobilization stress-induced oxidative and nitrosative stress in rats. Material and Methods:Thirty-two Wistar albino rats were randomly divided into four groups: control (i.p. physiological saline), SBT (i.p. 200 mg/kg/48h SBT extract), stress (i.p. physiological saline; 6-h cold plus immobilization stress) and SBT+stress (i.p. 200 mg/kg/48h SBT; 6-h cold plus immobilization stress). In liver and brain tissues 3-nitrotyrosine levels were determined by ELISA while total antioxidant capacity, total thiol, total glutathione, total nitrite+nitrate levels, superoxide dismutase and glutathione peroxidase activities were measured using colorimetric methods.Results:In the SBT+stress group, the total glutathione levels and glutathione peroxidase activities were significantly higher in both tissues, whereas the total nitrite+nitrate levels and superoxide dismutase activities decreased compared with the stress group. The 3-nitrotyrosine levels as oxidative and nitrosative stress markers were found to be significantly higher in SBT+stress group in both tissues than in the control. No significant differences were found between the stress and SBT+stress groups in the liver.Conclusion:The results show that SBT has antioxidant properties against cold plus immobilization stress-induced oxidative and nitrosative stress and that it can be recommended as a natural antioxidant and nutritional supplement.

Corresponding author : Mustafa Koray Gümüştaş