Turkish Neurosurgery
Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?
Necati KAPLAN1, Numan KARAARSLAN2, Ibrahim YILMAZ3, Duygu Sirin Yasar4, Feride Sinem Akgun5, Tezcan Caliskan2, Abdullah Talha Simsek2, Hanefi Ozbek3
1Istanbul Rumeli University, Corlu Reyap Hospital, Neurosurgery , Tekirdag,
2Namik Kemal University School of Medicine, Neurosurgery, Tekirdag,
3Istanbul Medipol University School of Medicine, Medical Pharmacology, Istanbul,
4Namik Kemal University, Faculty of Arts and Sciences, Molecular Biology and Genetics, Tekirdag,
5Istanbul Maltepe University School of Medicine, Emergency Medicine, Istanbul,
DOI: 10.5137/1019-5149.JTN.24336-18.0
Aim:In this study, it was aimed to investigate the effect of dabigatran, a new oral anticoagulant, on human primary cell cultures isolated from the intact intervertebral disc tissue. Material and Methods:Cell cultures were prepared from the tissues obtained from six cases underwent surgery because of spinal trauma. Except for the control group, dabigatran active pharmacological agent was applied to intact annulus fibrosus / nucleus pulposus primary cell cultures. After performing cell viability, toxicity and proliferation tests to all cultures in the control and study groups, the surface morphologies of the samples were evaluated. Subsequently, chondroadherin (CHAD) gene, cartilage oligomeric matrix protein (COMP), matrix metalloproteinase (MMP)-13 and -19 expressions were measured with real-time polymerase chain reaction (RT-PCR). The obtained data were analyzed statistically. Results:In the proliferation assays performed on the 20th day, cells in the dabigatran supplemented group were reported to have lost their viability by 46.37% compared to the control group. Expressions of all genes examined except MMP-13 were evaluated in the control group by time but, in contrast to the control group COMP and MMP-19 gene expressions were decreased in the dabigatran treated group. Besides, there was no expression of CHAD and MMP-13 in these cultures. Conclusion:The risk that a systemically applied drug accumulates in tissues and negatively affects the tissues and the micro-structures surrounding them should never be forgotten.
Corresponding author : Numan KARAARSLAN