Turkish Neurosurgery
Effects of Nigella Sativa and Nimodipine on Oxidative Stress and Apoptosis in Serum and Brain Tissue in Rats with Experimental Head Trauma.
Kaan Kamasak1, Ekinci Adnan2
DOI: 10.5137/1019-5149.JTN.25523-19.3

Aim:The aim of this study was to investigate whether Nimodipine and Nigella sativa oil (NS) have protective, antioxidant effects against brain injuries caused by experimental head trauma.Material and Methods:Fifty Winstar albino rats were randomly divided into 5 groups and experimental head trauma was applied to the rats. Okdidative parameters were compared in serum and brain tissue in rats. In addition, apoptosis and caspase-3 immunoreactivity were evaluated during histopathological examinations.Results:The Serum TAS levels were significantly increased in Nimodipine group (N) and Nimodipine + Nigella sativa group (N + NS) groups compared to controls. (p = 0.001, p <0.01). The tissue TAS levels were higher in the Nigella sativa group (NS) and N+NS groups compared to controls (p = 0.001, p <0.01). There was no significant difference in serum TOS levels between the groups (P=0.154). The TOS levels of the brain tissue were significantly higher in the NS group than the control group (p = 0.021). Conclusion:Nimodipine and NS have been shown to cause a significant reduction in the occurrence of oxidative stress in secondary brain injury in head trauma. We also found that histopathological apoptosis decreased after N, NS and N+NS treatment after head trauma.

Corresponding author : Kaan Kamasak