Turkish Neurosurgery
Effects of Nigella Sativa and Nimodipine on Oxidative Stress and Apoptosis in Serum and Brain Tissue in Rats with Experimental Head Trauma.
Kaan Kamasak1, Ekinci Adnan2
1HİTİT UNİVERSİTESİ, BEYİN CERRAHİ, ÇORUM,
2HİTİT UNİVERSİTY, OTOLARYNGOLOGY, ÇORUM,
DOI: 10.5137/1019-5149.JTN.25523-19.3

Aim:The aim of this study was to investigate whether Nimodipine and Nigella sativa oil (NS) have protective, antioxidant effects against brain injuries caused by experimental head trauma.Material and Methods:Fifty Winstar albino rats were randomly divided into 5 groups and experimental head trauma was applied to the rats. Okdidative parameters were compared in serum and brain tissue in rats. In addition, apoptosis and caspase-3 immunoreactivity were evaluated during histopathological examinations.Results:The Serum TAS levels were significantly increased in Nimodipine group (N) and Nimodipine + Nigella sativa group (N + NS) groups compared to controls. (p = 0.001, p <0.01). The tissue TAS levels were higher in the Nigella sativa group (NS) and N+NS groups compared to controls (p = 0.001, p <0.01). There was no significant difference in serum TOS levels between the groups (P=0.154). The TOS levels of the brain tissue were significantly higher in the NS group than the control group (p = 0.021). Conclusion:Nimodipine and NS have been shown to cause a significant reduction in the occurrence of oxidative stress in secondary brain injury in head trauma. We also found that histopathological apoptosis decreased after N, NS and N+NS treatment after head trauma.

Corresponding author : Kaan Kamasak