Aim:Strontium (Sr) compounds and glycyrrhiza glabra (licorice, G glabra) both have many applications and have been the subject of numerous studies. Although the therapeutic effects of Sr and G glabra have been investigated in other tissues, there is little information available on their neurotoxicity. Material and Methods:In this study, we conducted neurotoxicity assays on the human cortical neuronal cell line HCN-2 (CRL-10742) to determine the potential neurotoxic effects of Sr and G glabra on humans. Results:No significant decrease in HCN-2 cell viability was observed with longer exposure or concentrations up to 2000 µg/mL of Sr. The IC50 values of Sr for 24 and 48 hours of exposure were >2000 µg/mL, and 936.9 ± 0.09 µg/mL for 72 hours. However, we observed a significant reduction in HCN-2 cell viability with longer exposure and higher concentrations of G glabra. The IC50 values of G glabra for 24, 48, and 72 hours were 545.1 ± 0.03 µg/mL, 398.1 ± 0.03 µg/mL, and 393.3 ± 0.02 µg/mL, respectively. The results of this study suggest that Sr and G glabra are not neurotoxic. Conclusion:Additional studies are needed to further investigate the neurotoxicity of Sr and G glabra and elucidate the pathway by which these compounds exert their therapeutic effects in pathological conditions