Aim:ADAMTS-1 is the first described matrix metalloproteinase considered to be involved in the stages of cancer progression, including cell migration, invasion, and apoptosis. The present study analyzed the expression of ADAMTS-1, NF-κB, and STAT3 in human pleomorphic xanthoastrocytoma specimens and their correlation with glioma advancement.
Material and Methods:Pleomorphic Xanthoastrocytoma tumor cell lines were treated with low and high doses of cytokines at 24 and 48 hours to replicate the inflammatory environment. The effects of IL-1 were assessed with the scratch wound-healing assay, and the expression levels of ADAMTS-1, NF-κB, and STAT3 of the groups were determined by western blot analysis.
Results:Cytokine treatment significantly increased the migration of PXA glioma cells after scratching at 24h and 48h timepoints. Similarly, 10 and 30 ng/mL IL-1 induced 1.86 and 1.94 fold increases, respectively, in ADAMTS-1 expression after 24h, and 3 and 3.27 fold increases, respectively, after 48h, compared with the non-treatment control group.10 and 30 ng/mL IL-1 doses caused 2.5 and 2.6 fold increase, in NF-κB protein levels after 24h, and 3.16 and 3.41 fold increases after 48h, compared with the non-treatment group. The protein levels of STAT3 after 24h were 2.62 and 2.43 fold higher, and 3.78 and 3.84 fold higher after 48 hours, with 10 and 30 ng/mL IL-1, compared with the non-treatment group.
Conclusion:The proliferation and progression of glioma cells were proportional to the increased expression levels of ADAMTS-1, NF-κB, and STAT3. Our findings indicate that the proteolytic function of ADAMTS-1 may be associated with the malignant transformation of low-grade gliomas.