Turkish Neurosurgery
Role of Adamts-1 in Pleomorphic Xanthoastrocytoma tumor cells progression
Aysun Gokce1, Emre Cemal Gokce2, Asuman Sunguroglu3
1University of Health Sciences, Diskapi Yildirim Beyazit Training and Research Hospital, Department of Pathology, Ankara,
2University of Health Sciences, Ankara Oncology Training and Research Hospital, Department of Neurosurgery, Ankara,
3School of Medicine, Ankara University, Department of Medical Biology, Ankara,
DOI: 10.5137/1019-5149.JTN.31011-20.3

Aim:ADAMTS-1 is the first described matrix metalloproteinase considered to be involved in the stages of cancer progression, including cell migration, invasion, and apoptosis. The present study analyzed the expression of ADAMTS-1, NF-κB, and STAT3 in human pleomorphic xanthoastrocytoma specimens and their correlation with glioma advancement. Material and Methods:Pleomorphic Xanthoastrocytoma tumor cell lines were treated with low and high doses of cytokines at 24 and 48 hours to replicate the inflammatory environment. The effects of IL-1 were assessed with the scratch wound-healing assay, and the expression levels of ADAMTS-1, NF-κB, and STAT3 of the groups were determined by western blot analysis. Results:Cytokine treatment significantly increased the migration of PXA glioma cells after scratching at 24h and 48h timepoints. Similarly, 10 and 30 ng/mL IL-1 induced 1.86 and 1.94 fold increases, respectively, in ADAMTS-1 expression after 24h, and 3 and 3.27 fold increases, respectively, after 48h, compared with the non-treatment control group.10 and 30 ng/mL IL-1 doses caused 2.5 and 2.6 fold increase, in NF-κB protein levels after 24h, and 3.16 and 3.41 fold increases after 48h, compared with the non-treatment group. The protein levels of STAT3 after 24h were 2.62 and 2.43 fold higher, and 3.78 and 3.84 fold higher after 48 hours, with 10 and 30 ng/mL IL-1, compared with the non-treatment group.Conclusion:The proliferation and progression of glioma cells were proportional to the increased expression levels of ADAMTS-1, NF-κB, and STAT3. Our findings indicate that the proteolytic function of ADAMTS-1 may be associated with the malignant transformation of low-grade gliomas.

Corresponding author : Aysun Gokce