Turkish Neurosurgery
EVALUATION OF THE EFFECT OF GRANULOCYTE–MACROPHAGE COLONY STIMULATING FACTOR ON SPINAL FUSION IN A RAT MODEL OF SPINAL SURGERY
MEHMET FATİH AKSAY2, EMRE BAL 1, BEKİR ERAY KILINC1, AHMET ONUR AKPOLAT1
1University of Health Sciences, Fatih Sultan Mehmet Training and Research Hospital, Orthopaedics Surgery and Traumatology , İstanbul/Bostancı,
2Agrı Government Hospital, Orthopaedics Surgery and Traumatology , Ağrı,
DOI: 10.5137/1019-5149.JTN.44636-23.2
Aim:To evaluate the effect of granulocyte–macrophage colony stimulating factor (GM-CSF) on spinal fusion based on radiological, histopathological, and manual examination results in rats.Material and Methods:A total of 21 rats were used in this study. They were divided into the following three groups of seven rats each: preoperative GM-CSF, postoperative GM-CSF, and control. L4–L5 posterolateral fusion was performed in all three groups. While the preoperative GM-CSF group received 5 μg/kg GM-CSF subcutaneously for 5 days in the preoperative period, the postoperative GM-CSF group received the same intervention in the postoperative period, and no additional procedure was performed after surgery in the control group. All rats were sacrificed at 6 weeks, and the fusion site was evaluated using manual palpation and radiological and histopathological examinations.Results:The groups were compared according to the evaluation methods, manual examination, direct radiography, and histopathological examination scores as well as new bone volume determined via three-dimensional computed tomography were found to be higher in the preoperative GM-CSF and postoperative GM-CSF groups than in the control group (p <0.05).Conclusion:Our findings revealed that preoperative and postoperative GM-CSF administration had a positive effect on spinal fusion in rats undergoing spinal surgery.
Corresponding author : AHMET ONUR AKPOLAT