Turkish Neurosurgery 2021 , Vol 31 , Num 3
Effects of Benidipine Hydrochloride on Ischemia Reperfusion Injury of Rat Brain
Tayfun CAKIR1,Seyho Cem YUCETAS2,Gulce Naz YAZICI3,Mukadder SUNAR4,Yusuf Kemal ARSLAN5,Halis SULEYMAN6
1Erzincan Binali Yildirim University School of Medicine, Department of Neurosurgery, Erzincan, Turkey
2Adiyaman University School of Medicine, Department of Neurosurgery, Adiyaman, Turkey
3Erzincan Binali Yildirim University School of Medicine, Department of Histology and Embryology, Erzincan, Turkey
4Erzincan Binali Yildirim University School of Medicine, Department of Anatomy, Erzincan, Turkey
5Erzincan Binali Yildirim University School of Medicine, Department of Biostatistics, Erzincan, Turkey
6Erzincan Binali Yildirim University School of Medicine, Department of Pharmacology, Erzincan, Turkey
DOI : 10.5137/1019-5149.JTN.27372-19.3 AIM: To evaluate the neuroprotective effects of benidipine hydrochloride on the cerebral cortex tissues in rats exposed to cerebral ischemia-reperfusion (I/R) injury.

MATERIAL and METHODS: Twenty-four male Wistar albino rats were randomly divided into three groups, and administered benidipine hydrochloride (10 ?g/kg/day) orally through a catheter for 2 h to form the study group (BIR group, n=8). The I/R procedure was performed in the rats of the IR group (n=8), and a sham group was formed to determine the normal structure of the cerebral cortex (n=8). Transient ischemia was performed by clamping the left common carotid artery for 2 h. Subsequently, reperfusion was applied for 12 h. Cerebral infarct volumes were measured and cerebral cortex tissue samples were analyzed histopathologically and biochemically by measuring malondialdehyde (MDA), total glutathione, cyclooxygenase 1 (COX-1), COX-2 and superoxide dismutase (SOD)

RESULTS: The infarct area was markedly reduced in the BIR group vs. the IR group. Histopathologically, neuroprotective effects of benidipine hydrochloride were observed in the cerebral cortex tissues. The mean malondialdehyde and COX-2 levels were statistically higher in the IR group; however, in the BIR group, these levels were within the normal limits. Furthermore, the mean total glutathione, COX-1 and SOD levels were markedly lower in the IR group, and within the normal limits in the BIR group.

CONCLUSION: Benidipine hydrochloride may play a certain protective role in cerebral I/R injury. This effect may be related to improvement in the antioxidant capacity of brain tissue, and the inhibition of overproduction of inflammatory cytokines. Keywords : Cerebral ischemia reperfusion, Neuroprotection, Benidipine hydrochloride, Malondialdehyde, Glutathione, Rat

Corresponding author : Tayfun CAKIR, tayfuncakir198375@gmail.com