Turkish Neurosurgery
Low-level miR-199 contribute to neuropathic low back pain via TRPV1 by regulating the production of pro-inflammatory cytokines on macrophage
Li Zi1, Li Yonghong2, Li Zuohua3
1 The Sixth Hospital of Wuhan, Affiliated Hospital of Jianghan University, Orthopedics and Plastic surgery,, Wuhan,
2Wuhan Hanyang Hospital, Wuhan University of Science and Technology, Orthopedics, Wuhan,
3Taikang Tongji (Wuhan) Hospital, Orthopedics, Wuhan,
DOI: 10.5137/1019-5149.JTN.42453-22.2

Aim:Neuropathic low back pain (NLBP) directly affects the spine and lower limbs of patients. TRPV1 plays an important role in NLBP. However, the regulation mechanism of TRPV1 is still unclear. In this study, we aimed to explore the post-translational regulation of TRPV1.Material and Methods:qPCR was used to examine the gene mRNA levels. Western blot was used to examine the protein level. NLBP rat model was established for confirming what we observed in clinical samples. Dual-luciferase assay was used to verify the miR-199 targets on the 3’UTR of TRPV1. Cell coculture was used to explore the interaction between macrophages and nerve cells.Results:We found the mRNA level of TRVP1 decreased in the sinuvertebral nerve biopsy of NLBP. With bioinformatics prediction, miR199 would involve the post-transcription regulation of TRPV1. As the prediction, the miR199 level decreased in the clinical samples. Correlation regression analysis showed a negative correlation between miR-199 and TRPV1. The same phenomenon was confirmed in the rat NLBP model. With dual-luciferase assay, we confirmed that miR199 directly binds to the 3’UTR of TRPV1. Through co-culture of macrophage (THP1) and sNF96.2, we found that up or down-regulates miR-199 in macrophage and sNF96.2 could relieve or aggravate the injury of nerve cells strain.Conclusion:These results suggest that the occurrence of NLBP may be caused by the lower expression of miR-199 in macrophages and nerves via TRPV1.

Corresponding author : Li Zuohua