Turkish Neurosurgery 2016 , Vol 26 , Num 1
Single Operation with Simplified Incisions to Build an Experimental Cerebral Aneurysm Model by Induced Hemodynamic Stress and Estrogen Deficiency in Rats
Cong WU1, Yi LIU1, Min HE1, Lei ZHU2, Chao YOU1
1Sichuan University, West China Hospital, Department of Neurosurgery, Chengdu, Sichuan Province, China
2Sichuan University, Department of Human Anatomy, Chengdu, Sichuan Province, China
DOI : 10.5137/1019-5149.JTN.8748-13.4 AIM: To implement a surgical technique consisting of simplified incisions that allows all required procedures to be performed in one single operation for the purpose of reducing surgical stress in experimental animals.

MATERIAL and METHODS: Experimental animals (rats) were assigned to one of four groups: Group 0 was the (normal) control group, Group 1 consisted of rats that had an operation using multiple incisions, Group 2 consisted of rats who received a midline incision and were raised for 3 months, and Group 3 consisted of rats who also received a midline incision, but had been raised for 6 months. Rat blood pressure was measured by tail cuff method. The surgical characteristics and outcomes of the rats in Groups 1 and 2 were compared. Aneurysmal lesions of both branching and non-branching sites were compared amongst the 4 groups by observation of the cerebral vascular corrosion casts through a scanning electron microscope. Histological analyses of the induced aneurysms were performed.

RESULTS: The simplified incision technique significantly reduced the length of surgery and need for anesthesia redose during the operation. No aneurysms formed in the normal control rats. The incidence of saccular aneurysm formation significantly increased in Group 3. Histological analyses confirmed the aneurysms induced in the rats shared the same characteristics as human aneurysms.

CONCLUSION: Our modified surgical method reduced the surgical stress in rats. It also successfully induced both saccular and fusiform cerebral aneurysms. While longer incubation duration for aneurysm formation could be applied in future researches. Keywords : Cerebral aneurysm, Animal model, Oophorectomy, Etiology, Rats

Corresponding author : Chao You, chaoyou_wc@yahoo.com