Turkish Neurosurgery
Neuroprotective Effects of Pregabalin Against Spinal Cord Ischemia-Reperfusion Injury in Rats
Burak Kazanci1, Selcuk Ozdogan2, Ramazan Kahveci3, Emre Cemal Gokce4, Kazim Yigitkanli5, Aysun Gokce6, Bulent Erdogan4
1Ufuk University Faculty of Medicine, Neurosurgery, Ankara,
2Dr.Lutfi Kırdar Kartal Training and Research Hospital, Neurosurgery, Istanbul,
3Çanakkale State Hospital, Neurosurgery, Çanakkale,
4Turgut Özal University Faculty of Medicine, Neurosurgery, Ankara,
5Polatlı State Hospital, Neurosurgery, Ankara,
6Dışkapı Yıldırım Beyazıt Training and Research Hospital, Pathology, Ankara,
DOI: 10.5137/1019-5149.JTN.17959-16.1
Aim:We aimed in this study to evaluate the effect of pregabalin pretreatment on spinal cord ischemia-reperfusion(I/R) injury and compare it with methylprednisolone(MP).Material and Methods:Rats were randomized into four groups as follow: group 1 (sham)(n=8), group 2 (ischemia only)(n=8), group 3 (30 mg/kg pregabalin)(n=8), and group 4 (30 mg/kg methylprednisolone)(n=8). Laparotomy was performed without aortic clamp in the sham group. All animals were killed at 24 h after surgery. The spinal cord tissue samples were harvested and caspase-3 activity, tumor necrosis factor-alpha (TNF-α) and Interleukin-1 Beta (IL-1β) levels, catalase (CAT) activity, glutathione peroxidase (GPx) activity, superoxide dismutase (SOD) levels malondialdehyde (MDA) levels and nitric oxide (NO) levels were analyzed to investigate the effects of different excitatory and inflammatory pathways in mechanism of I/R injury. Ultrastructural histopathological and examinations were carried out. Neurological recovery was measured by Basso, Beattie, Bresnahan (BBB) test and Inclined Plane Test.Results:We were able to observe decresead caspase-3 activity and decraesed inflammatory markers like TNF-α, IL-1β, and decresaed excitotatory pathways like CAT, GPx, MDA, NO and SOD both in Pregabalin pretreatment and MP treatment groups. Pregabalin pretreatment produced better ultrastructural results compared to MP treatment, as with histopathological examination. Pregabalin group showed better recovery compared to MP treament group according to BBB scoring system.Conclusion:Pregabalin pretreatmet and MP treatmnet both has neuroprotective affect on IR injury by decreasing caspase dependant apoptosis, and inflammatory and oxidative stress markers and pregabalin pretreatment had better clinical effect compared to MP treatment.
Corresponding author : Selcuk Ozdogan