Turkish Neurosurgery
New clues in the malignant progression of glioblastoma: can thioredoxin system play a role?
Fatih Erdi1, Bulent Kaya1, Hasan Esen2, Yasar Karatas1, Sıddıka Fındık2, Fatih Keskin1, Bahadır Feyzioglu3, Erdal Kalkan1
1Necmettin Erbakan University Meram Faculty of Medicine, Neurosurgery, Konya,
2Necmettin Erbakan University Meram Faculty of Medicine, Pathology, Konya,
3Necmettin Erbakan University Meram Faculty of Medicine, Microbiology, Konya,
DOI: 10.5137/1019-5149.JTN.18991-16.2
Aim:The main aim of this study is to evaluate and compare the expression of thioredoxin reductase 1 (TrxR1) in primary and secondary glioblastoma samples.Material and Methods:Surgically resected human glioblastoma samples from 40 patients who underwent surgery at our institute were extracted from their histopathological specimens and divided into three groups. Ten histopathologically regular cerebral tissue, which acquired from the non-neoplastic portion of the specimens were assigned as the control group. Twenty specimens which include tumoral tissue from each type of glioblastoma. (WHO grade IV, primary and secondary) assigned as the primary and secondary glioblastoma groups. TrxR1 expression was analyzed by using both quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry. Isocitrate dehydrogenase 1 (IDH1) mutation was analyzed by immunohistochemistry. Ki-67 proliferative index and apoptosis were analyzed by immunohistochemistry. The results were compared between groups and the correlation between these parameters was analyzed. Results:The expression of TrxR1 and Ki-67 values were significantly high in primary glioblastoma. IDH1 mutation was significantly high in secondary glioblastoma. TrxR1 expression was found to be highly correlated with Ki-67 index. Apoptotic index was similar between primary and secondary glioblastoma.Conclusion:This study shows high TrxR1 expression in primary glioblastoma which can refer the role of Trx system in promoting the malignant progression by some complex processes.
Corresponding author : Fatih Erdi