Aim:As an oncogene, Golgi phosphoprotein 3 (GOLPH3) gene promotes migration and invasion in glioma cells. Vasculogenic mimicry (VM) formation implies exacerbation in glioma patients. However, the relationship between GOLPH3 and VM has not previously been clarified in gliomas. Here, we revealed an interaction between GOLPH3 and VM in glioblastoma cellsMaterial and Methods:Glioma tissues from 40 glioma patients with different pathological grades were collected. GOLPH3 and VM were evaluated by immunostaining in glioma tissues. Then, the correlation between GOLPH3 and VM were analyzed clinically. Additionally, a GOLPH3-downregulation lentivirus was constructed and transfected into the human primary glioblastoma cell line, U-87 MG. Afterwards, apoptosis, migration and invasion were assessed to determine the effects of downregulation GOLPH3. Then, E-cadherin and matrix metalloproteinase 2 (MMP2) were detected for assessment of the epithelial mesenchymal transition (EMT)Results:GOLPH3 and VM were found to be positively correlated following clinical analysis (P < 0.01, r = 0.788).Furthermore, GOLPH3 downregulation suppressed the migration and invasion of U87 MG cells (P <0.05), followed by upregulation of E-cadherin and downregulation of MMP2Conclusion:Collectively, our results demonstrate that GOLPH3 promoted VM in glioblastoma cells and that the corresponding mechanism was associated with the EMT. Our finding suggest that GOLPH3 may represent a promising therapeutic target for mitigating the recurrence and invasion of gliomas