Turkish Neurosurgery 2016 , Vol 26 , Num 6
14-3-3zeta Positive Cells Show More Tumorigenic Characters in Human Glioblastoma
Zhonghua LUO1,2, Xiaoliang YANG3, Lian-Ting MA1, Zhiqun WU2, Guozheng XU1, Xiaofeng WANG3, Weidong CAO4
1Wuhan General Hospital of Guangzhou Military Command, Department of Neurosurgery, Wuhan, China
2Tangdu Hospital of Fourth Military Medical University, Department of Interventional Radiology, Xi’an, China
3The 3rd Hospital of People Liberation Army, Department of Neurosurgery, Baoji, China
4Fourth Military Medical University, Xijing Hospital, Institute of Neurosurgery, Xi’an, China
DOI : 10.5137/1019-5149.JTN.13720-14.1 AIM: Expression of 14-3-3zeta is upregulated in many cancer types and plays an important role in tumorigenesis. Our previous studies have shown that 14-3-3zeta has a positive expression and is associated with a poor prognosis in patients with glioblastoma. In this study, we investigated whether 14-3-3zeta positive cells show more tumorigenic character and stronger chemotherapy resistance.

MATERIAL and METHODS: Six human glioblastoma cells lines were derived from the 6 patients with tumor, and the cells were sorted by 14-3-3zeta expression. The cell viability, invasion, tumorigenic ability and chemotherapy resistance were compared between the 14-3-3 positive and negative expression groups.

RESULTS: 14-3-3zeta positive cells displayed oncogenic properties, more tumorigenic character, high invasiveness, tumorsphere formation ability and resistance to temozolomide chemotherapy treatment.

CONCLUSION: Cells with 14-3-3zeta positive expression show more tumorigenic character and should be administered other treatments in the future. Keywords : 14-3-3zeta, Glioblastoma, Temozolomide, Tumorigenic

Corresponding author : Lianting Ma, liantingma@163.com