E-ISSN: 1019-5157
ISSN: 2651-5024
Research
HIPPOCAMPAL NEURONAL DAMAGE IN RATS EXPOSED TO A DOUBLE HIT: IRRADIATION AND HYPERTHERMIA
Gozde Erkanlı Senturk✉ ,
Cihan İsler ,
Rahşan Kemerdere ,
Mustafa Onur Ulu ,
Cenk Umay ,
Aydan Ozkaynakci ,
Rezzan Gülhan Aker ,
Feriha Ercan ,
Çiğdem Özkara ,
Filiz Onat ,
Mustafa Uzan
DOI: 10.5137/1019-5149.JTN.4012-10.1
Article in Press
Corresponding Author:
Gozde Erkanlı Senturk (gozdeerkanli@yahoo.com)
Abstract
Aim
In utero irradiation models induce diffuse neuronal damage. Experimental studies have shown that hyperthermia induced seizures are easily elicited and have high mortality accompanied by neuronal loss. Neuronal damage and loss are the results of cell death coupled with cortical development in altered cellular development. Aim of the study was to investigate the changes in hippocampus which was exposed to irradiation and hyperthermia.
Material and Methods
Four groups were studied: 1) Irradiation group was exposed to 225 cGy irradiation on the 17th gestational day; 2) Hyperthermia group was exposed to hyperthermia on the 10th postnatal day; 3) Hyperthermia plus irradiation group was exposed to in utero irradiation and postnatal hyperthermia; 4) Control group was sham operated. Animals were examined 3 and 6 months later.
Results
The hippocampus was atrophic with neuronal loss in CA regions and ectopic neurons were in irradiation group. Severe damage with the most atrophy was demonstrated in all regions of the irradiation plus hyperthermia group. In long term, damage was severe in all groups.
Conclusion
This study demonstrated more damage in hippocampi exposed to both irradiation and hyperthermia which may be taken as an evidence for the double hit hypothesis in the development of hippocampal damage.
In utero irradiation models induce diffuse neuronal damage. Experimental studies have shown that hyperthermia induced seizures are easily elicited and have high mortality accompanied by neuronal loss. Neuronal damage and loss are the results of cell death coupled with cortical development in altered cellular development. Aim of the study was to investigate the changes in hippocampus which was exposed to irradiation and hyperthermia.
Material and Methods
Four groups were studied: 1) Irradiation group was exposed to 225 cGy irradiation on the 17th gestational day; 2) Hyperthermia group was exposed to hyperthermia on the 10th postnatal day; 3) Hyperthermia plus irradiation group was exposed to in utero irradiation and postnatal hyperthermia; 4) Control group was sham operated. Animals were examined 3 and 6 months later.
Results
The hippocampus was atrophic with neuronal loss in CA regions and ectopic neurons were in irradiation group. Severe damage with the most atrophy was demonstrated in all regions of the irradiation plus hyperthermia group. In long term, damage was severe in all groups.
Conclusion
This study demonstrated more damage in hippocampi exposed to both irradiation and hyperthermia which may be taken as an evidence for the double hit hypothesis in the development of hippocampal damage.
Keywords
hippocampus
hyperthermia
irradiation
neuronal damage
rat