E-ISSN: 1019-5157
ISSN: 2651-5024
Research
Effect of Electrical Capacitance-Based Therapy on the Viability of U87 and T98-G Glioblastoma Cell Lines
Eray Abat✉ ,
Hatice Bekci ,
Mukerrem Betul Yerer ,
Nimetullah Alper Durmus ,
Ali Sahin ,
Serhat Albayrak ,
Sukru Oral ,
Ahmet Kucuk ,
Halil Ulutabanca
Beyin ve sinir cerrahisi, Erciyes Üniversitesi Tıp Fakültesi Hastaneleri; Neurosurgery, Erciyes University School of Medicine; Neurosurgery, Erciyes University; MOLEKÜLER BİYOLOJİ VE GENETİK, Erciyes University
DOI: 10.5137/1019-5149.JTN.49048-25.3
Article in Press
Corresponding Author:
Eray Abat (eray_abat@hotmail.com)
Abstract
Aim
: Glioblastoma is one of the most aggressive and lethal primary brain tumors, with survival remaining poor despite advances in multimodal therapy. In recent years, electric-fieldbased interventions have emerged as innovative approaches for the management of glioblastoma. Electro-capacitive cancer therapy (ECCT), a non-invasive technique that applies alternating electric fields through contactless capacitive electrodes, has demonstrated promising therapeutic potential. This study aimed to investigate the selective cytotoxic effects of ECCT on human glioblastoma cell lines (U87-MG and T98-G) and to assess its impact on normal epithelial cells.
Material and Methods
: U87-MG and T98-G glioblastoma cells, together with Beas-2B normal epithelial cells, were exposed to ECCT at a frequency of 100 kHz and 20 V for 24 h. Cell viability was measured using the MTT assay, and morphological changes were evaluated through phase-contrast microscopy. Statistical analyses were performed using one-way analysis of variance and Students t test, with significance defined as p < 0.05.
Results
Electro-capacitive cancer therapy exposure resulted in a time-dependent reduction in the viability of both glioblastoma cell lines. U87-MG cells exhibited significant growth inhibition at all exposure durations, whereas T98-G cells showed a marked decrease only after 24 h (p < 0.0001). In contrast, Beas-2B cells maintained normal morphology and viability, indicating that ECCT selectively targets malignant cells while sparing normal epithelial cells.
Conclusion
ECCT significantly suppresses glioblastoma cell proliferation without adversely affecting normal cells, underscoring its potential as a non-invasive adjunctive treatment for glioblastoma. Further preclinical and clinical studies are warranted to elucidate its underlying mechanisms, optimize exposure parameters, and determine its integration into multimodal glioblastoma therapy.
: Glioblastoma is one of the most aggressive and lethal primary brain tumors, with survival remaining poor despite advances in multimodal therapy. In recent years, electric-fieldbased interventions have emerged as innovative approaches for the management of glioblastoma. Electro-capacitive cancer therapy (ECCT), a non-invasive technique that applies alternating electric fields through contactless capacitive electrodes, has demonstrated promising therapeutic potential. This study aimed to investigate the selective cytotoxic effects of ECCT on human glioblastoma cell lines (U87-MG and T98-G) and to assess its impact on normal epithelial cells.
Material and Methods
: U87-MG and T98-G glioblastoma cells, together with Beas-2B normal epithelial cells, were exposed to ECCT at a frequency of 100 kHz and 20 V for 24 h. Cell viability was measured using the MTT assay, and morphological changes were evaluated through phase-contrast microscopy. Statistical analyses were performed using one-way analysis of variance and Students t test, with significance defined as p < 0.05.
Results
Electro-capacitive cancer therapy exposure resulted in a time-dependent reduction in the viability of both glioblastoma cell lines. U87-MG cells exhibited significant growth inhibition at all exposure durations, whereas T98-G cells showed a marked decrease only after 24 h (p < 0.0001). In contrast, Beas-2B cells maintained normal morphology and viability, indicating that ECCT selectively targets malignant cells while sparing normal epithelial cells.
Conclusion
ECCT significantly suppresses glioblastoma cell proliferation without adversely affecting normal cells, underscoring its potential as a non-invasive adjunctive treatment for glioblastoma. Further preclinical and clinical studies are warranted to elucidate its underlying mechanisms, optimize exposure parameters, and determine its integration into multimodal glioblastoma therapy.
Keywords
Apoptosis
Cell viability
Electric field therapy
Electro-capacitive cancer therapy
Glioblastoma